Product Name: GENLABS FOXO4-DRI 10mg Research Peptide Kit (Including 1x 10mg FOXO4-DRI Lyophilized Powder & 1x 10ml Bacteriostatic Water)
CAS Number: [To be filled by GENLABS - e.g., 2212441-64-0]
Molecular Formula: Câ‚₈₇H₃â‚â‚€N₅₆O₅₃S
Sequence: D-Y-R-K-L-L-Q-K-R-K-R-Q-Q-R-R-P-P-S-C*
Purity: ≥98% (as verified by HPLC-MS)
Storage: Store lyophilized powder at -20°C or below. Reconstituted solution should be stored at 2-8°C and used within a specified research timeframe.
In the advancing field of biogerontology and cellular biology, the study of cellular senescence represents a cornerstone for understanding age-related physiological decline. Senescent cells, characterized by their irreversible cell cycle arrest and the secretion of pro-inflammatory factors (the Senescence-Associated Secretory Phenotype, SASP), accumulate with age and contribute significantly to tissue dysfunction. GENLABS introduces FOXO4-DRI 10mg, a highly specific, research-grade peptide designed to disrupt a key protein-protein interaction involved in senescence maintenance. This product, provided as a complete kit with bacteriostatic water, offers scientists a precise and reliable tool for in vitro and pre-clinical investigation into senolytic strategies—the selective clearance of senescent cells.
FOXO4-DRI (FOXO4-D-Retro-Inverso) is a rationally designed, cell-permeable peptide with a novel retro-inverso structure. This design confers enhanced stability and bioavailability in biological systems compared to its linear counterpart. Its primary target is the forkhead box O4 (FOXO4) transcription factor and its interaction with the tumor suppressor protein p53.
In senescent cells, p53 is typically sequestered in the nucleus by FOXO4. This interaction is a critical mechanism that prevents p53 from initiating apoptosis (programmed cell death) in the senescent cell. FOXO4-DRI functions as a competitive inhibitor:
Specific Binding: The peptide's structure mimics the domain on FOXO4 that binds to p53.
Displacement: By binding with high affinity to p53, FOXO4-DRI displaces FOXO4, breaking the inhibitory complex.
p53 Activation & Apoptosis: Once freed from FOXO4, p53 can migrate to the mitochondria and initiate the intrinsic apoptotic pathway, leading to the selective elimination of the senescent cell.
Selectivity: This mechanism is preferentially active in cells where the FOXO4-p53 interaction is a dominant survival signal—primarily senescent cells—potentially offering a targeted senolytic effect while sparing healthy, proliferating cells in research models.
GENLABS FOXO4-DRI is intended for laboratory research use only. It serves as a critical reagent in the following potential areas of study:
Senolysis and Age-Related Research: Investigating the direct clearance of senescent cells from various tissues (e.g., dermal fibroblasts, endothelial cells, hepatocytes) in model systems and observing subsequent tissue function.
SASP Modulation Studies: Assessing the downstream effects of senescent cell removal on the inflammatory microenvironment by measuring changes in SASP factors like IL-6, IL-1α, MMPs, and others.
Healthspan and Function Studies: Exploring correlations between senolytic interventions and improvements in physiological function, resilience to stress, and tissue regeneration capacity in research models.
Model System Development: Utilizing FOXO4-DRI as a positive control or benchmark compound when validating new senolytic agents or cellular senescence assays.
Mechanistic Biology: Further elucidating the intricate signaling networks between FOXO proteins, p53 dynamics, and cell fate decisions in the context of stress and aging.
Exceptional ≥98% Purity: Every batch is rigorously analyzed via High-Performance Liquid Chromatography and Mass Spectrometry (HPLC-MS), ensuring minimal impurities and maximum consistency for your experimental results.
Complete Research Kit: Each order contains one 10mg vial of lyophilized FOXO4-DRI peptide and one 10ml vial of sterile, pH-balanced Bacteriostatic Water (0.9% Benzyl Alcohol). This provides everything needed for sterile reconstitution, eliminating sourcing variables.
Stable Retro-Inverso Design: The D-amino acid, reverse-sequence structure offers superior resistance to proteolytic degradation compared to L-peptides, leading to longer activity and more reliable data in cellular and pre-clinical research settings.
Sterile, Lyophilized Format: The peptide is sterile-filtered and lyophilized in a cleanroom environment to ensure stability, longevity, and readiness for precise reconstitution.
Commitment to Research Integrity: GENLABS provides comprehensive supporting documentation, including Certificate of Analysis (CoA) with lot-specific purity data and MS/HRMS verification. Our products are designed solely for use in controlled laboratory settings by qualified researchers.
Reconstitution Protocol:
Gently swirl the bacteriostatic water vial.
Aseptically, draw the desired volume of bacteriostatic water.
Slowly inject the water down the side of the FOXO4-DRI vial to avoid aggressive foaming.
Gently swirl or roll the vial until the lyophilized cake is fully dissolved. Do not vortex aggressively.
The reconstituted peptide solution should be clear and colorless.
Typical Research Dosage & Storage:
In Vitro Concentrations: Research often utilizes concentrations ranging from 1-10 µM, though optimal dosing must be empirically determined for each specific cell type and assay.
Post-Reconstitution Storage: The reconstituted solution is stable for approximately 30 days when stored refrigerated at 2-8°C. For long-term stability, aliquoting and storage at -20°C or -80°C is recommended. Avoid repeated freeze-thaw cycles.
Lyophilized Powder: Store unopened vials at -20°C or below for long-term stability (up to 24 months).
INTENDED USE STATEMENT: GENLABS FOXO4-DRI 10mg is sold strictly as a research chemical for laboratory and scientific investigation purposes only. It is not for human or veterinary use, not for ingestion, injection, or any form of personal consumption. It is not a drug, dietary supplement, or cosmetic ingredient. This product has not been evaluated or approved by the FDA or any other regulatory body for the diagnosis, treatment, cure, or prevention of any disease or condition.
Safety: Researchers must handle all peptides using appropriate laboratory safety protocols, including the use of personal protective equipment (PPE) such as gloves, lab coats, and eye protection. Assume all materials require sterile technique for cell culture applications.
Elevate your cellular senescence and longevity research with the precision and quality of GENLABS. Our FOXO4-DRI 10mg kit provides a reliable, high-purity tool to probe the frontiers of senolytic biology. Visit the GENLABS website to view the Certificate of Analysis for this product, explore our full portfolio of research peptides, and place your order.
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